Transmission Electron Microscopes (TEM)

JEOL JEM-Z200FSC (CRYO ARM™ 200) Field Emission Cryo-Electron Microscope

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JEOL JEM-Z200FSC (CRYO ARM™ 200) Field Emission Cryo-Electron Microscope

Cryo-electron microscopy has been established as a method to enable observation of cells and biological molecules with no fixation and no staining. Owing to the recent rapid progress of hardware and software, this microscopy technique has become increasingly important as an atomic-scale structural analysis method. In addition, technologies that enable analysis of membrane proteins without crystallization have been developed, resulting in increased use of cryo-electron microscopy for drug discovery. Thus, installation of cryo-electron microscopes (cryo-EM) in universities and research laboratories is greatly accelerating. To meet the needs of cryo-EM users, JEOL has developed a new cryo-EM “CRYO ARM™ 200”, which automatically acquires image data for Single Particle Analysis over a long period of time.

Features

  1. Automated specimen exchange system
    The system is composed of a specimen stage to cool samples at liquid nitrogen temperature and a cryo-transfer system to automatically transfer the cooled samples to the cryo-stage. Liquid nitrogen is automatically supplied to the liquid nitrogen tank as required. This automated system features the storing of up to 12 samples and the exchange of arbitrary one or more samples while the rest of samples are kept cooled between the specimen stage and specimen exchange system. These unique capabilities enable flexible scheduling of microscopy.

  2. Cold field emission gun (Cold FEG)
    Cold FEG produces a high-brightness electron beam with very small energy spread, offering high coherency. Thus, the CRYO ARM™ 200 achieves high resolution, high contrast imaging.

  3. Incolumn energy filter (omega filter)
    Equipped with an incolumn energy filter (omega filter), the CRYO ARM™ 200 acquires energy filtered images and energy loss spectra. Zero-loss image acquired with the microscope provides high contrast with reduced chromatic aberration.

  4. Automated image acquisition software for Single Particle Analysis
    The CRYO ARM™ 200 incorporates automated software. The software allows for automated detection of holes on the specimen grid for efficient acquisition of Single Particle Analysis images.

Main instrument

Electron gun

Cold field emission gun (Cold FEG)

Accelerating voltage

200kV

Energy filter

In-column Omega energy filter

Maximum specimen tilt angle

± 70°

※ Schottky field emission gun can optionally be configured.

Specimen Stage / Automated specimen exchange system

Specimen stage

Coolant

Liquid nitrogen
Automated liquid-nitrogen filling system built-in

Specimen cooling temperature

100K or less

Temperature measurement position

Specimen, Cryo-shield, LN2 tank

Specimen movements


X、Y

Motor drive (movements: ±1 mm)
Piezoelectric elements (movements: ±0.5 μm)

Z

Motor drive (movements: ±0.2 mm)

Tilt-X

Motor drive (tilts: ±70°)

Rotation within the specimen plane

0° or 90°

Specimen exchange system

Air-lock
Automated cryo-transfer system built-in

Cooling temperature
(specimen exchange chamber)

105K or less

Specimen exchange cartridge

Up to 4 specimens can be changed at one time.

Specimen parking stage

Up to 12 specimens can be held.

Please note:

Subject to technical changes; errors excepted. All brand names that appear in the text are registered trademarks of the manufacturers.

Cryo-electron microscopy unveils the mysteries of life by investigating various molecular structures in the "deep freeze".

Keiichi Namba
Professor at the Graduate School of Frontier Biosciences, Osaka University

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