Biology and biotechnology are the subject of much attention by the media and the public, and encompass many different disciplines. The fields of application range from classical zoology and botany to the classification of complex protein structures. Biotechnology in particular is a typical interdisciplinary technology that is not only based on disciplines such as biology and biochemistry, but also encompasses physics, chemistry, process technology and materials sciences.
JEOL systems offer a multitude of processes for analysing solid, liquid or gaseous substances, enabling the efficient handling of the most diverse problems.
The resolution of conventional optical microscopes and x-ray tomography systems is insufficient to understand the structural information of biological samples on a micro- and nanoscale. With JEOL electron microscopes it is possible to image and three-dimensionally reconstruct not only the smallest of biological structures such as viruses, but also microscale objects such as synapses or cells.
Reconstruction of a bacteriophage (left) and synapses (right)
Source data: Journal of Structural Biology 177 (2012) 589–601 and JEOL News 51
These days, biocides are used extensively for pest control in agriculture. The quantitative determination of the toxic residues of the applied pesticides is of vital importance for the authorisation of agricultural products. JEOL high-resolution analytical instrument are capable of detecting biocides quickly and simply, even with the smallest of concentrations.
Mass spectrum of various herbicides
Source data: JEOL Ltd. / DART Application Notes, page 57
The surface of lotus leaves can serve as a model for self-cleaning surfaces. The exact characterisation of these surfaces is vital in order to be able to recreate them. The surface of lotus leaves comprises small wax tubes that can be easily destroyed during examination with an electron beam. JEOL therefore offers tailor-made solutions that thermally stabilise the samples in a controlled manner and thus prevent them from being destroyed by the observation.
Surface of a lotus petal. The wax tubes have a diameter of approx. 50 nm
Source data: JEOL (Germany) GmbH
Cryo electron microscopy and the analysis of protein structures has undergone drastic changes since the last years. This technique was awarded the Nobel Prize in Chemistry 2017 (https://www.nobelprize.org/nobel_prizes/chemistry/laureates/2017/advanced-chemistryprize2017.pdf).
With the introduction of fast and highly sensitive camera techniques as well as dedicated, automated electron microscopes resolutions of 2 Ångström and lower could be achieved under near to native conditions. This single particle analysis method (SPA) allows to efficiently determine the structure of non crystalline proteins in order to design new drugs or to resolve the fundamental functions of biochemical or molecular biological processes.
GroEL protein at 40k x magnification (pixel size 0.12 nm), detector: K2 summit, instrument: CRYO ARMTM200, Schottky 200kV, inset: reduced live FFT, zero-loss image with 20eV slit width
Source data: JEOL Ltd., University Osaka, Prof. Namba
DNA origami is a new technology in synthetic biology, or rather the disciplines of biochemistry and biophysics. It involves DNA molecules being turned into random two- and three-dimensional nanoforms. These synthetically folded DNA molecules are used as e.g. future biocompatible carriers for active substances or to produce nanomachines or nanorobots.
Negatively contrasted TEM image of synthesised DNA molecules
Source data: JEOL (Germany) GmbH, Technical University of Munich, Prof. Dietz and Klaus Wagenbauer.
Specific preparation methods must be used for the high-resolution imaging and analysis of biological samples in an electron microscope. Food stuffs and their constituents in particular can only be imaged artifact-free through active cooling. JEOL electron microscopes are therefore prepared as standard for the installation of cryogenic systems so that sensitive samples can be prepared externally, transferred in a cooled state and subsequently examined in a cryogenic mode of operation.
Electron microscope image of powdered milk
Source data: JEOL (Germany) GmbH, DIL Quakenbrück
Metal powders are used for producing metal ceramics, among other things, with the density and structure of the powder playing an important role. With JEOL microscopes it is possible to image the structure and material composition down to the last detail. JEOL supplies an established and powerful complete solution for simple, artifact-free preparation and high-resolution imaging and analytics.
SEM image of a metallic powder
Source: JEOL (Germany) GmbH
Magnetotactic bacteria orientate themselves along the Earth's magnetic field with the help of magnetite particles enveloped in a membrane. These organisms serve as a model for examining the complex processes of biomineralisation. Entities known as magnetosomes (comprising magnetite crystal and membrane) are also tested as carriers for active substances and for new forms of therapy in medicine (hyperthermia therapy). JEOL supplies combined and fully automated solutions for the high-resolution elementary classification of the individual constituents.
Element analysis (red rectangle) at 120 kV in S(TEM) on magnetotactic bacteria, magnetite chains (green)
Source data: JEOL (Germany) GmbH
Fossil fuels such as Diesel are highly complex mixtures of a wide range of linear, cyclic and aromatic hydrocarbons. Separating theses and thus unequivocally determining the constituents of the fuel is therefore of crucial importance. The combination of special, two-dimensional GC techniques allows JEOL systems to quickly deliver exact structural information.
Two-dimensional total ion current chromatogram (TICC) of diesel fuel by GCxGC/TOFMS; separation after boiling point (abscissa) and polarity (ordinate)
Source data: JEOL Ltd., JMS-T200GC AccuTOF GCx brochure, page 4
To achieve the high-resolution imaging and analytics of biological samples, it is often necessary to examine the sample in its native state. Thanks to the patented JEOL Aqua Cover, it is even possible to image moist or hydrated samples in a scanning electron microscope at low pressure.
Image of a water droplet on the surface of a rose petal
Source data: JEOL Ltd. (Aqua Cover presentation)
During the course of evolution, living organisms have developed a multitude of microscopic surface structures to be able to e.g. move forward over smooth surfaces like a gecko, or on water like a water strider. To characterise these structures faithfully, JEOL supplies fully integrated solutions for artifact-free preparation and high-resolution imaging.
SEM image of the femur of an arthropod.
Image source: JEOL (Germany) GmbH, applied research paper for TUM Weihenstephan, Zoology Department
As a widespread fuel, petroleum comprises a mixture of liquid hydrocarbons. It is very difficult to perform molecular analysis on it with standard mass spectrometric methods. JEOL systems are capable of determining the molecular weight without fragmentation in a quick, uncomplicated manner by soft ionisation.
Mass spectra of an n-alkane, imaged with field ionisation (top) and electron impact ionisation (bottom)
Source data: JEOL Ltd., 200GC-Petroleum_and_Petrochemical_Solutions brochure
The linking of light microscopy signals and electron-optical details allows, among other things, conclusions to be drawn regarding the exact location of proteins in specific sections of tissues. JEOL manufactures intuitive, multi-system complete solutions for combining fluorescence and high-resolution electron microscopy.
Thin section of a zebra fish: correlatively superimposed electron and fluorescence microscopic images
Source data: JEOL (Germany) GmbH, Centre for Regenerative Therapies, TU Dresden.
The colours of a butterfly wing are created by pigment or structural colours. Due to the delicate surface of the wing, it is mechanically impossible to prepare a cross-section. With JEOL preparation systems and scanning electron microscopes, even fragile, organic structures can become accessible and visible.
Image of a cross-section through a butterfly wing (Morpho)
Source data: JEOL Ltd., Cross Section Polisher brochure
As part of a biopsy, tissue is removed and subsequently examined under the microscope. Labels can also be used to pinpoint the location of active substances to be examined. JEOL not only supplies specially developed, automated systems for simple and high-constrast imaging and high-resolution and ultrastructural diagnostics. With highly sensitive JEOL EDX detectors, it is also possible to pinpoint the location of the labels used.
TEM image of a tissue section
Source: JEOL (Germany) GmbH, Demo Friedrich Baur Institut in Munich
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