Transmission Electron Microscopes (TEM)

JEOL JEM-3300 (CRYO ARM™ 300 II) Field Emission Cryo Electron Microscope


JEOL JEM-Z300FSC (CRYO ARM™ 300) Field Emission Cryo Electron Microscope

The JEM-Z300FSC (CRYO ARM™ 300), equipped with a cold field emission gun, an in-column Omega energy filter, a side-entry liquid nitrogen cooling stage and an automated specimen exchange system, is a cryo-electron microscope (cryo-EM) that enables observation of bio-molecules at cryo-temperature. The automated specimen exchange system features the storing of up to 12 samples. In addition, the system allows for the exchange of an arbitrary one or more samples, thus enabling flexible scheduling. Furthermore, the combined use of a newly-designed in-column Omega energy filter and a hole-free phase plate system dramatically enhances the contrast of TEM images of biological specimens.


  1. Automated specimen exchange system
    The system is composed of a specimen stage to cool samples at liquid nitrogen temperature and a cryo-transfer system to automatically transfer the cooled samples to the cryo-stage.

  2. Cold field emission gun (Cold FEG)
    With its cold field emission gun the CRYO ARM™ 300 II achieves high resolution and high contrast imaging.

  3. In-column energy filter (omega filter)
    Equipped with an in-column energy filter (omega filter), CRYO ARM™ 300 II acquires energy filtered images and energy loss spectra. Zero-loss image acquired with the microscope provides high contrast with reduced chromatic aberration.

  4. Automated image acquisition software for Single Particle Analysis
    The CRYO ARM™ 300 II incorporates automated software. The software allows for automated detection of holes on the specimen grid for efficient acquisition of single particle analysis images.

Main instrument

Electron gun

Cold field emission gun (Cold FEG)

Accelerating voltage


Energy filter

In-column energy filter (omega filter)

Maximum specimen tilt angle

± 70°

※ Schottky field emission gun can optionally be configured.

Specimen Stage / Automated specimen exchange system

Specimen stage


Liquid nitrogen
Automated liquid-nitrogen filling system built-in

Specimen movements


Motor drive (movements: ±1 mm)
Piezoelectric elements (movements: ±0.5 μm)


Motor drive (movements: ±0.2 mm)


Motor drive (tilts: ±70°)

Rotation within the specimen plane

0° or 90°

Specimen exchange system

Automated cryo-transfer system built-in

Specimen exchange cartridge

Up to 4 specimens can be changed at one time.

Specimen parking stage

Up to 12 specimens can be held.

Please note:

Subject to technical changes; errors excepted. All brand names that appear in the text are registered trademarks of the manufacturers.

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